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| Registros recuperados: 73 | |
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| Latchere, Oihana; Mehn, Vincent; Gaertner-mazouni, Nabila; Le Moullac, Gilles; Fievet, Julie; Belliard, Corinne; Cabral, Philippe; Saulnier, Denis. |
| Environmental parameters, such as food level and water temperature, have been shown to be major factors influencing pearl oyster shell growth and molecular mechanisms involved in this biomineralization process. The present study investigates the effect of food level (i.e., microalgal concentration) and water temperature, in laboratory controlled conditions, on the last stages of pearl mineralization in order to assess their impact on pearl quality. To this end, grafted pearl oysters were fed at different levels of food and subjected to different water temperatures one month prior to harvest to evaluate the effect of these factors on 1) pearl and shell deposition rate, 2) expression of genes involved in biomineralization in pearl sacs, 3) nacre... |
| Tipo: Text |
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| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00428/54007/55223.pdf |
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| Waqalevu, Viliame; Besson, Marc; Gache, Camille; Roux, Natacha; Fogg, Lily; Bertucci, Frédéric; Metian, Marc; Lafille, Marc-andre; Maamaatuaiahutapu, Moana; Gasset, Eric; Saulnier, Denis; Laudet, Vincent; Lecchini, David. |
| Advancement and diversification of the aquaculture industry is reliant on the development of captive breeding and rearing protocols for novel fish species. Orbicular batfish (Platax orbicularis), a major emerging species in Polynesian aquaculture, live in brackish and marine waters around coral reefs, and are highly prized by Pacific Island communities for their high-quality meat. The present study describes the larval growth of P. orbicularis from hatching until 16 days after hatching (DAH) using meristics and thyroid hormone (T3) quantification. Our study highlighted that metamorphosis of P. orbicularis is critical to their production in aquaculture, as found for other species. Levels of the thyroid hormone T3 in P. orbicularis reached a peak at 12 DAH... |
| Tipo: Text |
Palavras-chave: Larval rearing; Mariculture; Metamorphosis; South pacific countries; Coral reefs. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00656/76816/77989.pdf |
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| Binesse, J; Delsert, Claude; Saulnier, Denis; Champomier Verges, M; Zagorec, M; Munier Lehmann, H; Mazel, D; Le Roux, F. |
| Genomic data combined with reverse genetic approaches have contributed to the characterization of major virulence factors of Vibrio species; however, these studies have targeted primarily human pathogens. Here, we investigate virulence factors in the oyster pathogen Vibrio splendidus LGP32 and show that toxicity is correlated to the presence of a metalloprotease and its corresponding vsm gene. Comparative genomics showed that an avirulent strain closely related to LGP32 lacked the metalloprotease. The toxicity of LGP32 metalloprotease was confirmed by exposing mollusk and mouse fibroblastic cell lines to extracellular products (ECPs) of the wild type (wt) and a vsm deletion mutant (Delta vsm mutant). The ECPs of the wt induced a strong cytopathic effect... |
| Tipo: Text |
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| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4931.pdf |
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| Brizard, Raphael; Boudry, Pierre; Haffray, Patrick; Labbe, C.; Suquet, Marc; Robert, Rene; Masse, G.; Pepin, Jean-francois; Saulnier, Denis. |
| In order to improve oyster culture, many research programs try and propose new methods aiming at improving genetically the product performances and quality (selection, polyploidisation). As for terrestrial animal production, the continuity of the different works as well as the spreading of the genetical progress would be made easier by the development of conservation techniques of the selected genotypes. Cryopreservation, which is often used in selection programs of terrestrial animals or fish farming species, is not always available for bivalves. This technique would enable the conservation of the gametes of individuals selected for their high growth potential, their resistance to diseases and/or their quality for a smaller cost and less risks than the... |
| Tipo: Text |
Palavras-chave: Tetraploïde; Diploïde; Concervation; Spermatozoides; Cryoconservation; Génétique; Crassostrea gigas; Huîtres. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/acte-3339.pdf |
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| Cochennec-laureau, Nathalie; Levy, Peva; Saulnier, Denis; Fougerouse, Angelique. |
| L’importance socio-économique de l’activité perlière justifie que le Service de la Perliculture mette en place un réseau de surveillance pathologique de l’huître perlière Pinctada margaritifera en Polynésie française. Les objectifs de ce réseau sont de prévenir l’introduction ou l’apparition d’agents infectieux, de prévenir leur propagation à l’intérieur d’une zone d’élevage, d’étudier les moyens de limiter leur impact, de surveiller leur évolution et enfin de garantir les échanges entre les zones d’élevage. Cette première année a permis d’élaborer le schéma de fonctionnement du réseau et de vérifier sa faisabilité. Cinq zones de prélèvements ont été définies en fonction de critères d’accessibilité à la zone et de facilité d’acheminement des échantillons,... |
| Tipo: Text |
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| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/00168/27886/26141.pdf |
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| De Decker, Sophie; Normand, Julien; Duperthuy, Marylise; Boudry, Pierre; Saulnier, Denis. |
| Rearing of Crassostrea gigas is the most economically important aquaculture activity in France. Bacteria belonging to Vibrio genus constitute one of the most abundant bacterial group in marine ecosystems. Two Vibrio species, V. splendidus and V. aestuarianus, were associated with many cases of mortality events in reared C. gigas spat and juvenile oysters, commonly during summer. This summer mortality syndrome has been extensively documented as the result of complex interactions between pathogens, host and environmental conditions. This work aims to study the Vibrio-host interactions and their modulations according to the virulence of pathogens and the genetic and/or physiological parameters of the host. These factors will be characterized by in vitro and... |
| Tipo: Text |
Palavras-chave: Genetic; Pathogens; Interaction; Vibrio; Crassostrea gigas; Oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/acte-3430.pdf |
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| Goarant, Cyrille; Reynaud, Yann; Ansquer, Dominique; De Decker, Sophie; Saulnier, Denis; Leroux, Frederique. |
| A collection of 57 isolates of Vibrio nigripulchritudo from either diseased or healthy shrimp and from shrimp farms environment was studied in order to gain a better understanding of the epidemiology of this pathogen, notably isolated from two distinct shrimp disease complexes. Molecular typing using two different techniques, arbitrarily primed PCR (AP-PCR) and multi-locus sequence typing (MLST), studied together with experimental pathology data allowed a relevant epidemiological insight into this possibly emerging pathogen. Additionally, results obtained with the two molecular typing techniques were congruent and allowed discriminating the strains associated with the "Summer Syndrome" from strains isolated from other contexts, especially the other shrimp... |
| Tipo: Text |
Palavras-chave: Virulence; Epidemiology; Typing; MLST; AP PCR; Mariculture; Vibriosis. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1913.pdf |
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| Pernet, Fabrice; Barret, Jean; Le Gall, Patrik; Malet, Nathalie; Pastoureaud, Annie; Munaron, Dominique; De Lorgeril, Julien; Bachere, Evelyne; Vaquer, Andre; Huvet, Arnaud; Corporeau, Charlotte; Normand, Julien; Boudry, Pierre; Moal, Jeanne; Quere, Claudie; Quilien, Virgile; Daniel, Jean-yves; Pepin, Jean-francois; Saulnier, Denis; Gonzalez, Jean-louis. |
| This study was conducted in the Mediterranean area during a mass mortality event of Pacific oyster Crassostrea gigas. The objectives of this work were to increase our understanding of the mass mortality phenomena in Pacific oysters, to identify risky cultivation practises and to propose mitigation strategies. In the first part of this work, we followed environmental parameters of the Thau lagoon in relation with mortality events. Then, we compared mortality and growth parameters of oysters as a function of their origins, their development stages and cultivation sites in relation with energetic, reproduction and pathogens. Finally, we conducted transfer experiments where oysters maintained free of mortality in open sea were transferred in the Thau lagoon... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00002/11354/7927.pdf |
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| Pernet, Fabrice; Barret, Jean; Le Gall, Patrik; Lagarde, Franck; Fiandrino, Annie; Huvet, Arnaud; Corporeau, Charlotte; Boudry, Pierre; Quere, Claudie; Degremont, Lionel; Pepin, Jean-francois; Saulnier, Denis; Boulet, Hélène; Keck, Nicolas. |
| The objectives of this study are to increase our understanding of the mass mortality phenomena in Pacific oysters Crassostrea gigas in the Thau lagoon and to examine the effect of cultivation practises. Mortality events in 2009 and 2010 always coincide with simple or dual infections involving primarily the Herpes virus OsHV-I and secondarily the bacteria Vibrio splendidus, when seawater temperature is between 17°C and 24°C. In the Thau lagoon, mortality starts early May and last until fall with an interruption period when seawater temperature is higher than 24°C in July and August. Mortality of oysters varies as a function of zootechnical life-history, hydrodynamics and cultivation practises. First, mortality depends on whether or not oysters have been... |
| Tipo: Text |
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| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00043/15404/12764.pdf |
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| Saulnier, Denis; Pepin, Jean-francois; Guesdon, Stephane; Degremont, Lionel; Faury, Nicole; Haffner, Philippe; Renault, Tristan; Travers, Marie-agnes; Tourbiez, Delphine; Geairon, Philippe; Le Moine, Olivier; Seugnet, Jean-luc; Soletchnik, Patrick. |
| This study aims to improve understanding of causes of mortalities in oyster Crassostrea gigas in pertuis charentais related to environmental parameters and the kinetics of targeted infectious agents, such as bacteria Vibrio splendidus (group ) and V. aestuarianus and Ostreid herpesvirus OsHV-1. Two sampling campaigns were carried out on the site of Agnas in 2009 and 2010, before, during or following mortality events of spat from March to September. The approach is to detect and quantify in short time step infectious agents from tissues of farmed oysters placed on rack and in various samples taken nearby: native oysters and other species (wild mussels and barnacles), seawater and sediment. With physical and chemical automatic probes placed on the site,... |
| Tipo: Text |
Palavras-chave: Huître creuse; Crassostrea gigas; Mortalité; Virus herpès; Vibrions; Environnement; Pertuis Charentais; Pacific oyster; Crassostrea gigas; Mortality; Herpes virus; Vibrios; Environment; Pertuis Charentais. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00058/16920/14400.pdf |
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| Aguirre Guzman, G; Ascencio, F; Saulnier, Denis. |
| The pathogenicity of Vibrio penaeicida Strains KH-1 and AM101, their culture-free supernatant (CFS), and their protein fraction obtained by 40% of ammonium sulfate precipitation (PFs40) were assessed in experimental challenges against juvenile Litopenaeus vannamei. Live Vibrio cells, CFS, and PFs40 from the AM101 strain produced a significantly higher mortality (p < 0.05) compared to the KH-1 strain. Toxicity and median lethal doses (LD50) of Fast Protein Liquid Chromatography (FPLC) products were evaluated on L. vannamei. The first FPLC fraction sample (A) from PFs40 of the AM101 strain displayed LD50 values of 1.68 and 5.61 mu g protein ind.(-1), respectively. The second FPLC process from Fraction A showed a peak (A1) also with toxic effects to... |
| Tipo: Text |
Palavras-chave: Pathogenicity; Exotoxin; Shrimp; Litopenaeus vannamei; Cysteine protease; Vibrio penaeicida. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/publication-3618.pdf |
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| Arnaud, Sophie; Goyard, Emmanuel; Vonau, Vincent; Herbaut, C; Prou, Jean; Saulnier, Denis. |
| Most bivalves species of the genus Pinctada are well known throughout the world for production of white or black pearls of high commercial value. For cultured pearl production, a mantle allograft from a donor is implanted into the gonad of a recipient oyster, together with a small inorganic bead. Because of the dedifferentiation of cells during the first steps of the host oyster's immunological reaction, so far the fate of the graft and its exact role in the process of pearl formation could not be determined via classical histological methods. Here we report the first molecular evidence of the resilience of the graft in the recipient organism by showing that cells containing genome from the donor are still present at the end of pearl formation. These... |
| Tipo: Text |
Palavras-chave: Genotype; Pearl; Pearl sac; Grafting; Biomineralization; Pearl oyster. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-2456.pdf |
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| Le Moullac, Gilles; Soyez, Claude; Latchere, Oihana; Vidal-dupiol, Jeremie; Fremery, Juliette; Saulnier, Denis; Lo Yat, Alain; Belliard, Corinne; Mazouni-gaertner, Nabila; Gueguen, Yannick. |
| The pearl culture is one of the most lucrative aquacultures worldwide. In many South Pacific areas, it depends on the exploitation of the pearl oyster Pinctada margaritifera and relies entirely on the environmental conditions encountered in the lagoon. In this context, assessing the impact of climatic stressors, such as global warming and ocean acidification, on the functionality of the resource in terms of renewal and exploitation is fundamental. In this study, we experimentally addressed the impact of temperature (22, 26, 30 and 34 °C) and partial pressure of carbon dioxide pCO2 (294, 763 and 2485 μatm) on the biomineralization and metabolic capabilities of pearl oysters. While the energy metabolism was strongly dependent on temperature, results showed... |
| Tipo: Text |
Palavras-chave: Global change; Pearl oyster; Bioenergetic; Biomineralization; Pacific ocean; French Polynesia. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00332/44364/43969.pdf |
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| Mai, Tepoerau; Toullec, Jordan; Van Wynsberge, Simon; Besson, Marc; Soulet, Stephanie; Petek, Sylvain; Aliotti, Emmanuelle; Ekins, Merrick; Hall, Kathryn; Erpenbeck, Dirk; Lecchini, David; Beniddir, Mehdi A.; Saulnier, Denis; Debitus, Cecile. |
| Marine natural products isolated from the sponge Fascaplysinopsis cf reticulata, in French Polynesia, were investigated as an alternative to antibiotics to control pathogens in aquaculture. The overuse of antibiotics in aquaculture is largely considered to be an environmental pollution, because it supports the transfer of antibiotic resistance genes within the aquatic environment. One environmentally friendly alternative to antibiotics is the use of quorum sensing inhibitors (QSIs). Quorum sensing (QS) is a regulatory mechanism in bacteria which control virulence factors through the secretion of autoinducers (AIs), such as acyl-homoserine lactone (AHL) in gram-negative bacteria. Vibrio harveyi QS is controlled through three parallel pathways: HAI-1, AI-2,... |
| Tipo: Text |
Palavras-chave: Porifera; Marine natural products; Quorum sensing inhibitors; Antibiotic; Fascaplysinopsis cf reticulata. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00598/71052/69366.pdf |
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| Le Moullac, Gilles; Phillip De Laborie, Loic; Saulnier, Denis; Goarant, Cyrille; Dehasque, Marleen. |
| Anderson (1992) y Raa (1996) han escrito impresionantes críticas acerca del uso de inmunoestimulantes en acuacultura. La mayoría de los trabajos citados se refieren principalmente al cultivo de peces y pocos acerca del camarón. Así que este trabajo está enfocado al camarón y las pocas pruebas publicadas. Varios de los inmunoestimulantes y su manera de actuar sobre el sistema inmune en camarones se describen brevemente, así como los métodos usados para administrar las sustancias inmunoestimulatorias para camarón. La dificultad para experimentar en este campo está señalada con algunos ejemplos. |
| Tipo: Text |
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| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/00029/14011/11197.pdf |
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| Aguirre-guzman, Gabriel; Labreuche, Yannick; Ansquer, Dominique; Espiau, Benoit; Levy, Peva; Ascencio, Felipe; Saulnier, Denis. |
| The pathogenicity of two V. penaeicida strains, AM101 and KH-1, with different geographic origin, and V. nigripulchritudo strain AM102, were investigated in juvenile blue shrimp species Litopenaeus stylirostris. Alive bacteria and protein fractions (PFs) obtained from cell-free supernatants (CFS) were used in experimental challenges. Strains AM102, AM101, and KH-1 produced respectively 60, 54 and 12% mortality at 96h after infection using 104 cfu mL-1 of bacterial suspension. Exocellular toxin-like factors were evidenced in CFS from the New Caledonian strains (AM102 and AM101) but not the Japanese strain (KH-1). At 48h post injection of each CFS, mortality rates were respectively 96,98 and 5% when these strains were cultivated at 20°C, whereas only O,16... |
| Tipo: Text |
Palavras-chave: Pathogenicity; Exotoxin; Shrimp; Litopenaeus stylirostris; Vibrio nigripulchritudo; Vibrio penaeicida. |
| Ano: 2003 |
URL: http://archimer.ifremer.fr/doc/2003/publication-2807.pdf |
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| Mai, T.; Tintillier, Florent; Lucasson, Aude; Moriou, C.; Bonno, E.; Petek, S.; Magre, Kevin; Al Mourabit, A.; Saulnier, Denis; Debitus, C.. |
| Sponges are a rich source for investigation of bioactive small molecules. They have been mostly investigated for the search of new pharmacological models or therapeutic agents for the treatment of human diseases. Micro-organisms can also represent a virulent pathogen for marine invertebrates such as sponges, which need to protect themselves against these microbes. Sponges' self defence mechanisms involving dialogue molecules thus represent a pertinent research track for potent anti-infective and anti-biofilm activities such as quorum sensing inhibitors (QSIs). The investigation of the QSI crude extract of Leucetta chagosensis Dendy, 1863 led to the isolation of three new alkaloids, isonaamine D, di-isonaamidine A and leucettamine D, along with the known... |
| Tipo: Text |
Palavras-chave: Biofouling; Leucetta chagosensis; Leucettamine; Naamidine; Naamine; Quorum sensing; Quorum sensing inhibitors; Sponges; Vibrio harveyi. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00275/38597/37189.pdf |
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| Tintillier, Florent; Moriou, Céline; Petek, Sylvain; Fauchon, Marilyne; Hellio, Claire; Saulnier, Denis; Ekins, Merrick; Hooper, John N. A.; Al-mourabit, Ali; Debitus, Cecile. |
| Four new brominated tyrosine metabolites, aplyzanzines C–F (1–4), were isolated from the French Polynesian sponge Pseudoceratina n. sp., along with the two known 2-aminoimidazolic derivatives, purealidin A (5) and 6, previously isolated, respectively, from the sponges Psammaplysilla purpurea and Verongula sp. Their structures were assigned based on the interpretation of their NMR and HRMS data. The compounds exhibited quorum sensing inhibition (QSi) and antifouling activities against several strains of bacteria and microalgae. To our knowledge, the QSi activity of this type of bromotyrosine metabolite is described here for the first time. |
| Tipo: Text |
Palavras-chave: Sponge; Pseudoceratina; Quorum sensing inhibitory activity; Antifouling; Verongiida; Bromotyrosine. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00630/74200/73825.pdf |
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| Registros recuperados: 73 | |
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